ORGANIZATION OF CELLS SIMILAR TO TISSUE AND MACROSCOPICAL DESIGNS SIMILAR TO TISSUE OBTAINED BY MEANS OF CULTURE OF MACROMASS OF CELLS AND METHOD OF MACROMASS CULTURE Russian patent published in 2008 - IPC C12N5/06 C12N5/08 

FIELD: medicine.

SUBSTANCE: method of obtaining the live organization of cells of a similar tissue is revealed, that is the macromass culture including three-dimensional designs similar to tissue, without the aid of a skeleton or a foreign matrix. The cells are plated in a vessel for cell culture with high density per unit area in a range from 3.33×10⁵ to 3×10⁶ cells per cm². The three-dimensional organization of the cells received in the specified way similar to a tissue is described. When cultivating cell macromasses it is possible to receive the cells, organized in forms similar tissues without the aid of a matrix, and three-dimensional macroscopical structures similar to tissue. In the given work the organization similar to a tissue and macroscopical structures similar to tissue are obtained from fibroblasts of a skin, the osteogene cells received from fat stromal cells, chondrocytes and from osteoblasts. Thus any skeleton or a foreign matrix for obtaining of a tissue or any other agents is not used (except high density of cell plating) which promote formation of a tissue, tissues have completely cellular parentage.

EFFECT: present invention allows simplifying the method of obtaining of substitutes of a human body tissue.

13 cl, 24 dwg, 1 tbl