FIELD: medicine, microbiology.
SUBSTANCE: invention concerns biotechnology, in particular genetic engineering. The recombinant plasmid pT7ApckA::loxpcat is designed by cloning of an incomplete pckA-gene in pT7B1ie-vector. The plasmid contains a fragment of a pckA-gene which includes a gene steady against Chloramfenicolum and loxP-sites. By transformation of cells E.coli with a plasmide DNA pT7ApckA::loxpcat strain E.coli FTR2717-producer L-threonine is obtained.
EFFECT: increase of L-threonine output in presence of high concentration of glucose.
2 cl, 3 dwg, 2 tbl, 3 ex
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