METHOD FOR PREPARING L-THREONINE Russian patent published in 2006 - IPC C12N15/00 C12N15/11 C12N1/21 C12P13/08 C12R1/19

Abstract RU 2288265 C2

FIELD: biotechnology, biochemistry, amino acids, genetic engineering.

SUBSTANCE: invention relates to a method for producing L-threonine. Method involves partial inactivation of gene encoding enzyme threonine hydratase (tdc) in genome DNA of microorganism by using recombinant technology procedure. Strain of microorganism Escherichia coli pGm TN-PPC12 (KSMM-10236) is transformed with DNA fragment Δtdc::1oxpKan and the strain E. coli TRN212 (KSSM-1053) is prepared. This strain is cultured for preparing L-threonine. The claimed invention provides significant increasing the yield of L-threonine.

EFFECT: valuable properties of strain.

4 cl, 3 dwg, 4 tbl, 5 ex

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RU 2 288 265 C2

Authors

Pak Dzeh Uong

Li Buong Chon

Kim Deh Cheol

Li Dzin Kho

Cho Dzeh Uong

Pak Uong Khun

Dates

2006-11-27—Published

2002-05-16—Filed