FIELD: medicine.
SUBSTANCE: invention refers to medicine and veterinary science, namely to immunodiagnostics, and can be used in life-time serodiagnostics dirofilariasis in animals and human. The process involves producing the purified somatic antigen Dirofilaria repens by sequential mechanical and ultrasonic homogenisation combined with protein extraction in aqueous saccharose solution 0.25 M, followed by settling the supernatant fluid in cooled acetone and dissolving the vacuum-dried deposition in potassium-phosphate buffer.
EFFECT: method involves producing the high-specific and high-sensitive somatic antigen.
3 ex, 3 tbl, 1 dwg
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Authors
Dates
2009-05-27—Published
2008-01-09—Filed