FIELD: biotechnology.
SUBSTANCE: method involves covalent bonding of ligands of protein nature with a modified carrier. The carrier used is aluminium silicate, which contains magnetite. The carrier is modified by a 1-5% solution of carboxymethylated lignine. Subsequent activation of the sorbent is done using carbodiimide (N-cyclohexyl-N'-(2-morpholinoethyl)-carbodiimide-methyl-n-toluene sulphonate). Sensitivity of polarisation fluoroimmunoassay using magnoimmunosorbents is 101 m.t./ml on corpuscular antigen and 25-50 ng/ml on water-soluble antigen.
EFFECT: method allows for obtaining magnoimmunosorbents with high specific capacity.
1 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR IMMUNE SERUM SORPTION | 2000 |
|
RU2200324C2 |
| METHOD FOR IMMUNOSORBENT PRODUCTION | 2003 |
|
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| METHOD FOR PREPARING IMMUNOSORBENT | 2004 |
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| METHOD FOR OBTAINING MAGNETIC IMMUNOSORBENTS FOR SELECTIVE CONCENTRATION OF F. TULARENSIS FROM ENVIRONMENTAL OBJECTS WITH SUBSEQUENT DETECTION BY THE MALDI-TOF MS METHOD | 2020 |
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| METHOD FOR PREPARING MAGNETIC IMMUNOSORBENT FOR DETECTION OF BACTERIAL ANTIGENS | 2003 |
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RU2246968C2 |
| METHOD OF IMMUNOSORBENT PRODUCING (VARIANTS) | 1997 |
|
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| METHOD FOR CARRYING OUT IMMUNOENZYME ANALYSIS | 2005 |
|
RU2290641C1 |
| METHOD FOR PRODUCTION OF BIOMAGNETIC IMMUNOSORBENT FOR BACTERIAL ANTIGEN DETECTION (VARIANTS) | 2003 |
|
RU2271540C2 |
| METHOD FOR DIAGNOSTICS OF SYPHILIS (VARIANTS) | 1999 |
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RU2200327C2 |
| METHOD OF INDICATION OF MICROORGANISMS | 1999 |
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RU2165081C2 |
