METHOD FOR PRODUCTION OF BIOMAGNETIC IMMUNOSORBENT FOR BACTERIAL ANTIGEN DETECTION (VARIANTS) Russian patent published in 2006 - IPC G01N33/543 

FIELD: biotechnology, immunology, microbiology.

SUBSTANCE: freeze-dried 10-40 % cell slurries are treated singly or twice with 25-35 % iron sulfate solution. After repeat freeze drying treatment with 20-25 % ammonium aqueous solution is carried out. Then obtained magnetic cells are activated with 5 % glutaric aldehyde for 4 h. Further cells are treated with species-specific competent immunoglobulins from lemic, tularemia, or brucellosis serum containing 10 mg/ml of antibodies for 24±2 h. Method of present invention makes it possible to detect lemic bacteria, tularemia, and brucellosis in concentration of 10³-10⁴ m.c./ml. Obtained biomagnetic immunosorbents may be stored for 2 years at 4-8° without losses of specific activity.

EFFECT: biomagnetic immunosorbents of improved sensitivity and prolonged storage-time.

5 cl, 9 ex