FIELD: chemistry; biochemistry.
SUBSTANCE: expression of endogeneous reserve proteins in plant seeds is suppressed through transformation of plant cells using a genetic construct, which contains a seed-specific promoter, functionally linked with a DNA sequence which codes transcription factors of endogenous genes or part of these factors, including their different combinations. The transcribed target of the said sequence is capable of suppressing, slowing down or in some other way, lowering expression of reserve proteins of seeds in a plant cell. Further, this or another plant cell is transformed by the construct, which contains the same seed-specific promoter, functionally linked with the DNA sequence which codes the heterologous polypeptide of concern.
EFFECT: in a plant regenerated from the said cell, expression of endogenous mRNA is reduced, which enables accumulation of heterologous polypeptide in that plant.
25 cl, 5 dwg, 7 ex
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|---|---|---|---|
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|
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|
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| REGULATORY NUCLEIC ACID MOLECULES FOR ENHANCING SEED-SPECIFIC AND/OR SEED-PREFERENTIAL GENE EXPRESSION IN PLANTS | 2010 |
|
RU2692924C2 |
