FIELD: medicine.
SUBSTANCE: invention refers to medicine, and can be used to analyse water for its biological activity that is ensured by preparing standard saline of water. Dead-end nerve cell preparations are prepared and placed in saline for 40 minutes. It is exposed to electric stimulation with four after-threshold intensity pulse packets at frequency 100 Hz. Packet length is 5 seconds with the interval 5 seconds. It is followed with discharging and recording excitatory postsynaptic potential amplitude of N-methyl-D-aspartate-receptor both initial at the moment of stimulation and 5, 15, 30 and 40 minutes after stimulation. Biological activity of water is determined by comparing initial parametres of caused cell activity during incubation in water and thereupon considering duration of staying in water.
EFFECT: method allows analysing water of various sources for biological activity either prior to and after processing and comparing levels thereof.
2 tbl, 2 ex
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|
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Authors
Dates
2009-12-27—Published
2008-06-09—Filed