METHOD FOR BIOLOGICAL NEURAL NETWORK TRAINING (IN EXPERIMENT) Russian patent published in 2017 - IPC G09B23/28 C12N5/02 C12N5/79 

FIELD: medicine.

SUBSTANCE: methodincludes application of a microelectrode matrix to grow a culture of dissociated neuronal cells, stimulation of two sections of neuronal cells by high-frequency electric pulses based on the rule of the time-dependent pulse of synaptic plasticity and evaluation of the efficiency of the biological neural network training. A microelectrode matrix combined with a two-chamber microfluidic chip containing microchannels for culturing of two axon-associated populations of dissociated neuronal cells is used. Stimulation of two sections of neuronal cells by high-frequency electric pulses is performed on the basis of the rule of the time-dependent pulse of synaptic plasticity, in which the pulse is directed along the axons in the microchannels from the source population to the receiving population. Neural network areas corresponding to the location of pre- and postsynaptic neurons in the constructed communication are stimulated. Evaluation of the biological neural network training efficiency is perforned according to the direct indicator of a specified functional connection and indirect indicators.

EFFECT: invention allows directional modification of functional bonds in the culture of dissociated cells and detection of the induced effect based on their functional activity.

2 dwg, 2 ex