METHOD OF PREPARING PROBIOTIC DRUG Russian patent published in 2010 - IPC A61K35/66 A23K1/165 

FIELD: chemistry; biochemistry.

SUBSTANCE: method involves preparation of a stock culture through combined growth of lactic acid bacteria Enterococcus faecium B 3491, Lactobacillus plantarum B 3242, Bifidobacterium bifidum AC 1247 under submerged cultivation conditions, taken in ratio of 1:1:1 until achieving bio-titre of each bacterium of not less than 5·10⁸ CFU/ml. Growth is carried out in a liquid medium which is a mixture of cow milk and water in ratio of 1:1. A mixture of extraction cakes-aromatic herbs is prepared from cumin seeds, coriander seeds and parsley herbs after CO₂ extraction with the following ratio of components, wt %: extraction cake of cumin seeds 20-40, extraction cake of coriander seeds 30-50, extraction cake of parsley 30. The obtained mixture of extraction cakes is inoculated with the stock culture in ratio of 1:1 and incubated at 35-40°C for 4-6 days to obtain not less than 5·10⁹ CFU/g in a semihumid preparation. The obtained semihumid preparation is mixed with the obtained mixture of extraction cakes until attaining moisture content of 8-12% in the ready preparation and a bio-titre of each microorganism of not less than 5·10⁸ CFU/g.

EFFECT: increased safety and survival rate of young growth and increased body weight gain.

3 ex