METHOD FOR PREPARING DRY PROBIOTIC PREPARATION "BATSELL" Russian patent published in 2006 - IPC A61K35/66 A23K1/165 

FIELD: biotechnology, veterinary science.

SUBSTANCE: invention relates to technology for preparing bacterial preparations used in prophylaxis diseases in animals. Method involves the separate culturing under conditions of submerged culturing of microorganisms Bacillus subtilis VKPM B-8130, Lactobacillus acidophilus Scav. VKPM B-4625 and Rhuminococcus albus Kr. The grown mother cultures of B. subtilis VKPM B-8130, L. acidophilus Scav. VKPM B-4625 and R. albus Kr in the ratio = 1:1 and autoclaved sunflower grist is inoculated with this mixture in the following ratio of components, wt.-%: autoclaved sunflower grist, 45-55; mother culture of Lactobacillus acidophilus Scav. VKPM B-4625, Rhuminococcus albus Kr, 20-30, and mother culture of Bacillus subtilis VKPM B-8130, 20-30. The prepared mixture is incubated at temperature 30-45°C for 4-8 days to obtain 1 x 10⁸ cells/g of preparation in semi-wet preparation, and prepared preparation is mixed with sunflower grist in the ratio = (1:10)-(1:12) to obtain the preparation with residual moisture 8-10%. Invention provides enhancing effectiveness in preparing the dry probiotic preparation, reducing material consumptions in its production, simplifying technology for its preparing and using inexpensive raw in preparing the preparation based on exclusion the drying step from the technological schedule and preparing the preparation with higher titer value.

EFFECT: improved preparing method.

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