FIELD: medicine.
SUBSTANCE: invention relates to the preparation of genetically engineered construct for transgene expression in mammal cells in vitro and in vivo. The modified sequence of gene of angiopoietin-1 (Angl) is offered. The sequence is designed for transfection of mammal cells for expression of angiopoietin-1 therein. Production of angiopoietin-1 in case of using the optimised gene AGPopt is 1.6 times more than when using the natural gene hAGP. Herewith, the content of angiopoietin-1 in a culture medium of transfected cells makes not less than 0.5 mcg/ml.
EFFECT: invention can be used in medicine.
3 dwg, 1 tbl, 1 ex
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