HEMOPOIETIC PROTEIN (VARIANTS), DNA SEQUENCE, METHOD FOR PREPARING HEMOPOIETIC PROTEIN, METHOD FOR SELECTIVE EXPANSION OF HEMOPOIETIC CELLS, METHOD FOR PRODUCING DENDRITIC CELLS, METHOD FOR STIMULATING FOR PRODUCING HEMOPOIETIC CELLS IN PATIENT, PHARMACEUTICAL COMPOSITION, METHOD FOR TREATMENT Russian patent published in 2005 - IPC

FIELD: biotechnology, molecular biology, medicine, genetic engineering, pharmacy.

SUBSTANCE: the hemopoietic protein comprises the amino acid sequence of the formula: R₁-L₁-R₁, R₂-L₁-R₁, R₁-R₂ or R₂-R₁ wherein R₁ represents the modified ligand flt-3; R₂ represents the modified human IL-3, the modified or unmodified colony-stimulating factor. Modification of R₁ is carried out by addition of N-end with C-end directly or through linker (L₂) that is able to join N-end with C-end to form new C- and N-ends. The modified human IL-3 is prepared by replacing amino acids at positions 17-123. The human G-CSF is modified by exchange of amino acids. The hemopoietic protein is prepared by culturing cells transformed with vector comprising DNA that encodes the hemopoietic protein. The hemopoietic protein stimulates producing hemopoietic cells and this protein is used as a component of pharmaceutical composition used in treatment of humans suffering with tumor, infectious or autoimmune disease. Invention provides preparing multifunctional hemopoietic proteins eliciting the enhanced activity with respect to stimulation of hemopoietic cells and eliciting the improved physical indices. Invention can be used for preparing chimeric multifunctional hemopoietic proteins.

EFFECT: improved preparing and producing method, valuable medicinal properties of protein.

22 cl, 19 dwg, 18 tbl, 117 ex