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2 385 946

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IPC

C12Q1/68(2006-01-01)

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Application

2008151550/13, 2008-12-26

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Start date

2008-12-26

(22)

Actual filing date

2008-12-26

(45)

Published

2010-04-10

(72)

Inventor

Slobodenjuk Vladimir VladimirovichAleshkin Vladimir AndrianovichAfanas'Ev Stanislav StepanovichLapin Boris Arkad'EvichVoropaeva Elena AleksandrovnaBannov Vasilij AleksandrovichKostrova Ol'Ga MikhajlovnaKaraulov Aleksandr ViktorovichRubal'Skij Oleg Vasil'EvichDzhikidze Ehteri KapitonovnaVolozhantsev Nikolaj ValentinovichDjatlov Ivan AlekseevichSvetoch Ehduard Arsen'EvichGrechishnikova Ol'Ga Gennad'EvnaMetel'Skaja Valerija AlekseevnaBajrakova Aleksandra L'VovnaAfanas'Ev Maksim StanislavovichEgorova Ekaterina AleksandrovnaAfanas'Ev Denis StanislavovichUrban Julija NikolaevnaRubal'Skij Evgenij OlegovichKurakova Anna Alekseevna

(73)

Holder

Federal'Noe Gosudarstvennoe Uchrezhdenie Nauki Nauchno-Issledovatel'Skij Institut Ehpidemiologii I Mikrobiologii Imeni G.N. Gabrichevskogo Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka" Mniiehm Im. G.N. Gabrichevskogo Rospotrebnadzora)

DIAGNOSTIC TECHNIQUE FOR HUMAN OR SIMIAN CHLAMIDIA INFECTION AND KIT FOR IMPLEMENTATION THEREOF Russian patent published in 2010 - IPC C12Q1/68

Abstract RU 2385946 C1

FIELD: medicine.

SUBSTANCE: DNA is recovered from a clinical material or cell culture infected with Chlamydia. It is followed with multiplex PCR with using primers Ctr 5' -TGGCGATATTTGGGCATCC-3, Cpn 5'-CGGAATAATGACTTCGGTTG-3' and R 5'-CTTCTTTACCTGGTACGCTC-3, and electrophoretic analysis of the amplification products to diagnose: human or simian Chlamydia monoinfection caused by Chlamydia trachomatis in case of positive PCR with primers Ctr 5'-TGGCGATATTTGGGC ATCC-3' and R 5' -CTTCTTTACCTGGTACGCTC-3'; human or simian Chlamydia monoinfection caused by Chlamydophila pneumoniae in case of positive PCR with primers Cpn 5,-CGGAATAATGACTTCGGTTG-3' and R 5'-CTTCTTTACCTGGTACGCTC-3'; human or simian Chlamydia mixed infection caused by Chlamydia trachomatis and Chlamydophila pneumoniae in case of positive PCR with primers Ctr 5'-TGGCGATATTTGGGCATCC-3', Cpn 5'-CGGAATAATGACTTCGGTTG-3' and R 5 '-CTTCTTTACCTGGTACGCTC-3'.

EFFECT: invention can be used to identify the most common agents of Chlamydia infection with simplified diagnostic technique.

2 cl, 6 ex

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RU 2 385 946 C1

Authors

Slobodenjuk Vladimir Vladimirovich

Aleshkin Vladimir Andrianovich

Afanas'Ev Stanislav Stepanovich

Lapin Boris Arkad'Evich

Voropaeva Elena Aleksandrovna

Bannov Vasilij Aleksandrovich

Kostrova Ol'Ga Mikhajlovna

Karaulov Aleksandr Viktorovich

Rubal'Skij Oleg Vasil'Evich

Dzhikidze Ehteri Kapitonovna

Volozhantsev Nikolaj Valentinovich

Djatlov Ivan Alekseevich

Svetoch Ehduard Arsen'Evich

Grechishnikova Ol'Ga Gennad'Evna

Metel'Skaja Valerija Alekseevna

Bajrakova Aleksandra L'Vovna

Afanas'Ev Maksim Stanislavovich

Egorova Ekaterina Aleksandrovna

Afanas'Ev Denis Stanislavovich

Urban Julija Nikolaevna

Rubal'Skij Evgenij Olegovich

Kurakova Anna Alekseevna

Dates

2010-04-10—Published

2008-12-26—Filed