FIELD: medicine.
SUBSTANCE: DNA is recovered from a clinical material or cell culture infected with Chlamydia recovered from the clinical material. It is followed with multiplex PCR with primers Ctr 5'-TGGCGATATTTGGGCATCC-3, R 5'-CTTCTTTACCTGGTACGCTC-3, PLf 5'-TCCGGAGCGAGTTACGAAGA-3' and PLr 5' -AATCAATGCCCGGGATTGGT-3'. Then amplification products are exposed to electrophoretic analysis to predict: manifested human or simian Chlamydia infection in case of positive PCR with primers Ctr 5'-TGGCGATATTTGGGC ATCC-3, R 5' -CTTCTTTACCTGGTACGCTC-3', PLf 5'-TCCGGAGCGAGTTACGAAGA-3' and PLr 5'-AATCAATGCCCGGGATTGGT-3'; obliterated human or simian Chlamydia infection in case of positive PCR with primers Ctr 5'-TGGCGATATTTGGGCATCC-3', R 5'-CTTCTTTACCTGGTACGCTC-3' and negative PCR with primers PLf 5' -TCCGGAGCGAGTTACGAAGA-3' and PLr 5' -AATCAATGCCCGGGATTGGT-3'.
EFFECT: invention can be used to diagnose human or simian Chlamydia infection, to predict both manifested and obliterated human and simian Chlamydia infection caused by Chlamydia trachomatis with simplifying the diagnostic technique for Chlamydia infection.
2 cl, 3 ex
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Authors
Dates
2010-04-10—Published
2008-12-26—Filed