FIELD: medicine.
SUBSTANCE: solution of substance to be defined and a sample solution for comparison are prepared. As a diluent for the preparation of the test solutions 0.1 M solution of hydrochloric acid is used. As a sample for comparison yellow dimethyl is used. Optical density of solution of substance to be defined and of yellow dimethyl sample for comparison are measured by spectrophotometer at a wavelength of 318 nm. The results are calculated according to the formula with a conversion coefficient.
EFFECT: improving of results reproducibility; reducing of cost, complexity and analysis errors.
3 ex
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Authors
Dates
2010-05-10—Published
2008-04-30—Filed