FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to agricultural microbiology and can be used in microbiological industry for making various bacterial fertilisers. The method involves culturing bacteria on a solid Ashby medium and is distinguished by that free aerobic nitrogen fixers are first separated from liquid elective cultures and subsequently cultured into pure cultures on a solid culture medium. The most actively developing strains are separated from the pure cultures and transferred to a liquid culture medium which does not contain nitrogen. A microbiological preparation based on Pseudomonad bacteria genus and a soil filtrate are added to the obtained stable bacterial suspension which stimulates agricultural plant growth. The mixture is thoroughly stirred, the filtered Ashby medium for azotobacter and oligonitrophiles with a solution of Fedorov microelements is then added and culturing in a fermenter is carried out at 22-28°C with the mixer rotating at 180 rpm for 10 days - 4 times a day for two hours.
EFFECT: invention enables to obtain a stable, well replicated bacterial suspension on exclusively synthetic media.
1 tbl, 3 ex
