FIELD: biotechnology.
SUBSTANCE: invention relates to a method for culturing a diploid culture of fetal pigs larynx cells, sensitive to influenza A, influenza B viruses, respiratory syncytial virus (hRSv), adenovirus (hAdv). Said method comprises washing fetal pig larynx explants with Hanks' solution and performing tissue disaggregation, obtained cells are grown on a mixture of nutrient media with NuSera 10% serum substitute, formation of a continuous monolayer is obtained on 4th–5th day, the limit of cell line cultivation is 26 passages.
EFFECT: invention provides cultivation of a strain free from contamination with viruses, mycoplasmas, bacteria, fungi, cells of other animal species, which is a biosafe substrate for cultivating human viruses and providing a high level of reproduction thereof.
1 cl, 4 ex
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