METHOD OF HYSTAMINE RATE ASSESSMENT IN EPITHELIAL CELLS OF RATS ENDOMETRIUM WITHIN SEX CYCLE Russian patent published in 2010 - IPC G01N33/52 

FIELD: medicine.

SUBSTANCE: invention belongs to medicine, notably to morphology and hystochemistry. In this method of histamine rate assessment in epithelial cells of rats endometrium within sex cycle, sample acquisition and following fluorescent-histochemical analysis with orthophtalic aldehide is made. In each phase of sex cycle histamine rate assessment in vaginal content is made. Assessment's result is compared with rate of histamine which is 0.50±0.07 at early estrus stage, 0.34±0.04 at late estrus stage, 0.68±0.08 at metaestrus stage, 0.72±0.12 at early diestrus stage, 0.75±0.13 at late diestrus stage, 0.87±0.11 at proestrus stage. Measurement unit is conventional unit of measuring device. When rate of histamine in vaginal content is below control value the rate of histamine in epithelial cells of endometrium is assessed as reduced, if it exceeds control value same does the histamine rate in endometrial cells.

EFFECT: improvement of evaluation accuracy.

2 tbl, 2 ex