FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and specifically to culturing a lipase producer. The method involves preparation of a culture medium containing fish flour, sunflower oil, corn-steep extract, ammonium hydrophosphate and water in the given ratio. The prepared culture medium is inoculated with a water-spore suspension of a lipase producer in form of Rhizopus oryzae BKM F - 1403 and cultured with aeration and temperature of 30-32°C for 48 hours. After that triton X-305 is added to the culture medium in a given amount and then cultured again for 24 hours. At the end of culturing, mycelium and suspended particles are separated through filtration.
EFFECT: invention increases lipase activity.
2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING LIPOXYGENASE ENZYME PREPARATION | 2002 |
|
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| METHOD OF PREPARING AMYLOLYTIC AND PROTEOLYTIC ENZYME COMPLEX | 1993 |
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