FIELD: biotechnology, in particular deep cultivation of Bacillus circulans.
SUBSTANCE: invention relates to deep cultivation of pectin-lyase producer such as Bacillus circulans, liquid standardization thereof and drying of cultural liquid or filtrate thereof. Cultural broth contains maize flour (2.75-3.0 %) or maize starch (2.75 %) or mixture of maize flour (3 % or less) with wheal bran (4 % or less). Method includes pH changing of filtrate or cultural liquid standardized with salts before dehydration. Changing of broth composition males it possible to obtain increased productivity of pectin-lyase biosynthesis process (up to 34100 U/cm3, to increase enzyme heat resistance up to 85.1 % (based on starting level); and changing of pH value (from 6.0 to 7.8) makes it possible to additionally increase of heat resistance up to 12.2 %.
EFFECT: bacterial enzyme with increased heat resistance.
2 tbl, 4 ex
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