SET OF TWO PRIMERS FOR AMPLIFYING NUCLEIC ACID OF HEPATITIS A VIRUS, METHOD OF DETECTING HEPATITIS A VIRUS USING SAID SET (VERSIONS) AND KIT FOR DETECTING HEPATITIS A VIRUS Russian patent published in 2010 - IPC C12N15/51 C12Q1/68 

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology, particularly to a set of two primers for amplifying nucleic acid of hepatitis A virus, a method of detecting hepatitis A virus in a biological sample, as well as a kit for detecting hepatitis A virus in a biological sample. The method of detecting hepatitis A virus (HAV) in a biological sample which contains antibodies produced by an individual, is realised by bringing a solid substrate into contact with capturing nucleic acids containing one or more oligonucleotides. Further, the solid substrate is brought into contact with the biological sample under hybridisation conditions. The solid substrate is then separated from the sample. Further, the isolated nucleic acids are amplified with a set of two primers. Presence of amplified nucleic acids as an indicator of the presence or absence of HAV in the sample is then determined. The method can also involve determination of the presence of an amplified internal control sequence.

EFFECT: invention enables provides a sensitive, reliable diagnostic test for detecting hepatitis A virus (HAV) in biological samples from potentially infected individuals.

43 cl, 5 dwg, 1 tbl, 3 ex