FIELD: chemistry.
SUBSTANCE: invention discloses a novel method of producing separate Nε-nitroso-Nε-[(2-chloroethyl)carbamoyl]-L-lysine and Nε-[(2-chloroethyl)-N-nitrosocarbamoyl]-L-lysine, which employs a method for preparative high performance liquid chromatography in a reverse-phase version using a mobile phase in form of a solution isopropyl alcohol in water with concentration of 2-5% and rate of feeding the mobile phase of 4.0-8.0 ml/min, where the volume of the sample is not more than 2.6% of the volume of the column.
EFFECT: higher output of separate compounds.
1 cl, 5 ex
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