FIELD: medicine.
SUBSTANCE: recombinant protein Collbd-BMP-2 is described, consisting of collagen-binding domain Collbd (SPARC(w)), of extracellular calcium-dependent protein SPARC (VM-40 or osteonectin) of a human being and spacer sequence GGS. Method for its production is proposed, including growing of strain E. coli M15 [pREP4, pCollbd-BMP-2]. According strain and plasmid are proposed.
EFFECT: invention makes it possible to provide a high level of recombinant protein Collbd-BMP-2 production and simultaneously simple and efficient scheme for cleaning of this protein with its immobilisation on collagen-containing sorbent included into composition of new generation of items and materials of medical purpose - implants and coatings.
4 cl, 1 dwg, 3 ex
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RU2408730C1 |
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RU2401302C2 |
| RECOMBINANT PLASMID (VERSIONS), STRAIN ESCHERICHIA COLI (VERSIONS) - PRODUCER OF CHIMERIC PROTEINS, CHIMERIC PROTEIN (VERSIONS), METHOD OF IMMOBILISATION, CONCENTRATION AND PURIFICATION OF RECOMBINANT CELLULOSE PROTEINS, METHOD OF IMMOBILISATION OF RECOMBINANT POLYSTYRENE CARRIER PROTEINS | 2008 |
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| RECOMBINANT PLASMID pD4spGBD, Escherichia coli STRAIN - RECOMBINANT PROTEIN D4-GBD PRODUCER, RECOMBINANT PROTEIN D4-GBD AND METHOD FOR MAKING THEREOF, METHOD FOR STUDYING PROTEIN D4-GBD FIXATION TO SERUM ANTIBODIES, METHOD FOR MAKING SPECIFIC PROTEIN D4-GBD ANTIBODIES | 2008 |
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RU2401305C2 |
| RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION | 2010 |
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RU2422524C1 |
