FIELD: medicine.
SUBSTANCE: recombinant protein Collbd-CBD is described, consisting of collagen-binding domain Collbd (SPARC(w)), of extracellular calcium-dependent protein SPARC (VM-40 or osteonectin) of a human being and spacer sequence GGS. Method for its production is proposed, including growing of strain E. coli M15 [pREP4, pOC-Collbd]. According strain and plasmid are proposed.
EFFECT: invention makes it possible to provide a high level of recombinant protein Collbd-CBD production and simultaneously simple and efficient scheme for cleaning of this protein with its immobilization on collagen-containing sorbent included into composition of new generation of items and materials of medical purpose - implants and coatings.
4 cl, 1 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PROTEIN Collbd-BMP-2, RECOMBINANT PLASMID pCollbd-BMP-2, STRAIN Escherichia coli-PRODUCER OF RECOMBINANT PROTEIN Collbd-BMP-2, METHOD TO PRODUCE RECOMBINANT PROTEIN Collbd-BMP-2 | 2009 |
|
RU2408727C1 |
| RECOMBINANT PROTEIN Collbd-BMP-7, RECOMBINANT PLASMID pCollbd-BMP-7, STRAIN Escherichia coli-PRODUCER OF RECOMBINANT PROTEIN Collbd-BMP-7, METHOD TO PRODUCE RECOMBINANT PROTEIN Collbd-BMP-7 | 2009 |
|
RU2408730C1 |
| RECOMBINANT PLASMID, RECOMBINANT STRAIN, RECOMBINANT PROTEIN BMP-2 AND EXTRACTION METHOD OF RECOMBINANT PROTEIN IN DIMERIC FORM | 2012 |
|
RU2499048C1 |
| RECOMBINANT PLASMID, RECOMBINANT STRAIN, RECOMBINANT PROTEIN BMP-7 AND EXTRACTION METHOD OF RECOMBINANT PROTEIN IN DIMERIC FORM | 2012 |
|
RU2499047C1 |
| METHOD OF PRODUCTION OF PROTEIN-MINERAL COMPOSITION CONTAINING RECOMBINANT PROTEIN Collbd-BMP-2 | 2012 |
|
RU2492237C1 |
| RECOMBINANT PLASMID (VERSIONS), STRAIN ESCHERICHIA COLI (VERSIONS) - PRODUCER OF CHIMERIC PROTEINS, CHIMERIC PROTEIN (VERSIONS), METHOD OF IMMOBILISATION, CONCENTRATION AND PURIFICATION OF RECOMBINANT CELLULOSE PROTEINS, METHOD OF IMMOBILISATION OF RECOMBINANT POLYSTYRENE CARRIER PROTEINS | 2008 |
|
RU2378371C1 |
| RECOMBINANT PLASMID, Escherichia coli STRAIN, CHIMERIC PROTEIN AND THEIR APPLICATION | 2010 |
|
RU2422524C1 |
| RECOMBINANT PROTEIN LACspCBD POSSESSING BETA-GALCTOSIDASE ACTIVITY AND ABILITY FOR SPONTANEOUS BINDING WITH CELLULOSE-CONTAINING SORBENTS, RECOMBINANT PLASMID DNA ENCODING SYNTHESIS OF RECOMBINANT PROTEIN LACspCBD, STRAIN Escherichia coli M15 [pREP4, pLACspCBD] AS PRODUCER OF RECOMBINANT PROTEIN LACspCBD, METHOD FOR PREPARING IMMOBILIZED RECOMBINANT PROTEIN LACspCBD ON CELLULOSE AND METHOD FOR ENZYMATIC CLEAVAGE OF LACTOSE | 2004 |
|
RU2278160C2 |
| RECOMBINANT PLASMID DNA ENCODING TUL4spCBD RECOMBINANT PROTEIN SYNTHESIS, M15[pREP4, pTUL4spCBD] ESCHERICHIA COLI STRAIN AS PRODUCER OF TUL4spCBD RECOMBINANT PROTEIN, TUL4spCBD RECOMBINANT PROTEIN, METHOD FOR PRODUCTION THEREOF, AND METHOD FOR PRODUCTION OF SPECIFIC ANTIBODY AGAINST TUL4spCBD PROTEIN | 2004 |
|
RU2270249C1 |
| RECOMBINANT PLASMID pAg85A-CBD, Escherichia coli [pREP4, pAg85A-CBD] STRAIN, CHIMERIC PROTEIN Ag85A-CBD AND THEIR APPLICATION | 2010 |
|
RU2429292C1 |
