FIELD: medicine.
SUBSTANCE: method of monomer antibody recovery from a compound containing large molecular weight antibody aggregates provides contacting of the compound with hydroxyapatite resin and elution of purified antibody from resin. There are also disclosed versions of such method providing protein A affinity chromatography, ion-exchange chromatography and hydroxyapatite chromatography.
EFFECT: methods allow producing a compound with the lower content of large molecular weight antibody aggregates.
23 cl, 4 dwg, 14 tbl, 11 ex
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| METHOD FOR PURIFYING VITAMIN K DEPENDENT PROTEINS SUCH AS COAGULATION FACTOR IX | 2011 |
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