FIELD: biotechnologies.
SUBSTANCE: disclosed is method of purifying coagulation factor IX (FIX). Method involves chromatography using multimodal resin, at which is contact fraction containing FIX, with multimodal resin, flushing with flushing buffer to remove impurities and elution FIX with multimodal resin buffer containing arginine, with subsequent collection of fractions containing FIX in pure or enriched form. Disclosed method enables further use of step for cleaning of target protein, associated with multimodal resin before elution, as well as possibility of performing elution in mild conditions.
EFFECT: proposed method can be used in biochemistry for purifying coagulation factor IX.
16 cl, 1 dwg, 17 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PURIFYING VITAMIN K-DEPENDENT PROTEINS, SUCH AS COAGULATION FACTOR VII | 2011 |
|
RU2731720C2 |
| METHOD OF PURIFYING GROWTH FACTOR PROTEIN | 2011 |
|
RU2571926C2 |
| METHOD OF PURIFYING COAGULATION FACTOR VIII | 2009 |
|
RU2698392C2 |
| METHOD FOR PURIFICATION OF BLOOD-COAGULATION FACTOR VIII | 2009 |
|
RU2567811C2 |
| FUSION PROTEIN PURIFICATION METHOD | 2015 |
|
RU2698654C2 |
| METHOD OF PURIFYING COAGULATION FACTOR VIII PROTEIN AND METHOD OF STABILISING FACTOR VIII PROTEIN | 2008 |
|
RU2493163C2 |
| METHODS FOR PURIFICATION OF ADENO-ASSOCIATED VIRUSES | 2018 |
|
RU2785661C2 |
| PURIFICATION OF POLYPEPTIDES | 2009 |
|
RU2544860C2 |
| CONTINUOUS MULTISTEP PROCESS FOR PURIFYING ANTIBODIES | 2014 |
|
RU2662668C2 |
| PROTEIN PURIFICATION USING BIS-TRIS BUFFER | 2012 |
|
RU2603347C2 |
