FIELD: medicine.
SUBSTANCE: method involves sporangiospore cultivation on a solid nutrient medium with adding trehalose in an amount 0.5-1.5 % for 5-7 days. A terminal phase of spore formation includes green light exposure of wave length 530 nm and irradiation intensity 1.3-1.7 kWt/m2. A spore inoculum is used for separate cultivation of (+) and (-)Blakeslea trispora mycelia. It is followed with joint cultivation in a liquid nutrient medium with added sunflower-seed oil in the ratio of (+) and (-) mycelia as 1:7, a lycopin formation stimulator 2-amino-6-methyl-pyridine and a 0.10-0.20 % antioxidant 6-methyl-2-ethl-3-oxypyridine. The prepared lycopin-containing diomass is frozen-dried, ground, extracted in chloroform. The extracted extract are concentrated, mixed with sunflower-seed oil and encapsulated.
EFFECT: optimised lycopin biotechnology and higher yield of the biologically active agent.
6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF LYCOPINE PRODUCING | 1995 |
|
RU2102416C1 |
| METHOD OF LYCOPIN PREPARING | 1997 |
|
RU2115678C1 |
| (-)-STRAIN OF HETEROTHALLIC PHYCOMYCETUS BLAKESLEA TRISPORA PRODUCING LYCOPIN IN PAIR WITH DIFFERENT (+)-STRAINS OF BLAKESLEA TRISPORA AND METHOD FOR MICRIBIOLOGICAL SYNTHESIS OF LYCOPIN | 2001 |
|
RU2211862C2 |
| METHOD FOR OBTAINING BIOLOGICALLY ACTIVE PRODUCT | 2000 |
|
RU2166868C1 |
| METHOD FOR PREPARING LICOPIN, PHOSPHOLIPIDS, FATTY ACIDS AND ERGOSTEROL BY COMBINED CULTURING (+)= AND (-)-STRAINS OF FUNGUS Blakeslea trispora | 2004 |
|
RU2270868C1 |
| TWO STRAINS OF HETEROTHALLIC FUNGUS BLAKESLEA TRISPORA KP74 AND KP 86 PRODUCING BETA-CAROTENE | 2000 |
|
RU2177505C2 |
| METHOD OF PRODUCTION OF MYCELIAL MASS FOR BETA-CAROTENE PRODUCING | 1999 |
|
RU2177506C2 |
| PAIR STRAINS OF HETEROTHALLIC FUNGUS BLAKESLEA TRISPORA F - 674(+) AND F-551 (-) PRODUCING BETA-CAROTENE | 1993 |
|
RU2053301C1 |
| METHOD OF CRYSTALLINE LICOPIN PREPARING | 1996 |
|
RU2126806C1 |
| METHOD OF WOUND-HEALING PREPARATION PREPARING | 1994 |
|
RU2086247C1 |
