FIELD: medicine.
SUBSTANCE: method includes isolation of cells from cell material source. Application of isolated cells onto growth medium, as such biomaterial based on the basis of polymer of native form of gialuronic acid, in which immediately before placement of cells physiological solution (0.9 NaCl %) is added, liquid loss being compensated by addition of phosphate buffer solution each 1-1.5 hours. Cultivation of cells with formation of monolayer of isolated cells with each 1-1.5 hour further preparation of histologic preparations for control of cell viability.
EFFECT: invention makes it possible to obtain cell culture with preservation of their functional activity and formation of o cell monolayer in standard laboratory conditions.
11 dwg, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CELL CULTURE MATRIX | 2014 |
|
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| METHOD FOR PRODUCING VIABLE BREAST CELLS | 2009 |
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| METHOD OF CULTURING TUMOUR STEM CELLS OF GLIOBLASTOMA | 2014 |
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| HISTO-EQUIVALENT BIOPLASTIC MATERIAL | 2013 |
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| METHOD FOR SIMULATING TISSUE STRUCTURE OF HUMAN RETINA | 2012 |
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| METHOD OF SEPARATING FIBROBLASTS, METHOD OF CREATING THEREOF-BASED BIOTRANSPLANT (VERSIONS) AND METHOD OF REGENERATING HUMAN TISSUES (VERSIONS) | 2013 |
|
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| METHOD FOR MAKING DERMAL MATRIX | 2013 |
|
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| BIOTRANSPLANT FOR TREATMENT OF DEGENERATIVE AND TRAUMATIC DISEASE OF CARTILAGE TISSUE AND METHOD FOR ITS PREPARING | 2005 |
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RU2301677C1 |
