METHOD FOR SIMULATING TISSUE STRUCTURE OF HUMAN RETINA Russian patent published in 2013 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: eyeball is enucleated in autopsy, washed with an alcohol solution and Hank's solution with antibiotics. An anterior segment of the eyeball is removed along a dentate line. A vitreous body and neural retina are separated from a retinal pigment epithelium (RPE). That is followed by the devitalising treatment of the separated retina for creating a cell-free retinal frame. This frame is washed in a nutrient medium with antibiotics. Then, the frame is transferred into a slide chamber, and colonised with the RPE cells. For producing an RPE cell culture, an eyecup is filled with Hank's solution with EDTL. Then, the native RPE cells are collected. A part of the RPE cells is transferred into a sterile medium for recovery, and centrifuged. Then, the recovered cells are resuspended in a growth medium, pipetted, bottled and cultured with the medium changed to form a primary culture. Then, the primary culture cells are passaged. These cells are cultured in culture flasks. The passage 1-5 cultured cells are removed as monolayer cultures from the culture surface, and the cultured RPE cells are directed as a suspension for colonisation pf the cell-free retinal frame. In this case, retinal inspection and devitalising treatment control are conducted by microscopy. Monitoring the cell cultures is ensured by immune histochemical test, PCR and microscopy.

EFFECT: method provides creating a three-dimensional PRE cell culture model with the specified parameters of number, density and tissue differentiation due to phased controlling the modelling process.

14 cl, 2 ex, 3 tbl, 28 dwg