METHOD OF COMPLEX IMMUNOELECTROPHORETIC IDENTIFICATION OF SPOROUS BACTERIAL FORMS BY CAPILLARY ELECTROPHORESIS Russian patent published in 2011 - IPC C12Q1/02 G01N33/569 

FIELD: medicine.

SUBSTANCE: immunoglobulin work solution migration time specific to each series and manufacturer is set; specific immunoglobulin is used as a reference mark (control). Further, an analysed sample suspected for the presence of sporous bacteria is prepared for an antigen-antibody reaction by adding the specific immunoglobulin work solution in a phosphate buffer to the sample. In reaction, a complex of bacterium in the sporous form and specific immunoglobulin is produced. It is followed with capillary electrophoresis, UV detection, complex migration time test in the capillary electrophoresis system to be compared with the migration time in the capillary electrophoresis system of the specific immunoglobulin (control). If the complex migration time exceeds the specific immunoglobulin migration time by 50 seconds, the presence of bacteria in the sporous form is detected in the sample. Also, the analysis conditions and parametres are described.

EFFECT: invention allows the high-reliable specific identification of bacteria in the sporous form.

6 cl, 2 dwg, 1 ex