METHOD OF ESTIMATING ANTI-ERGOTYPIC RESPONSE IN EXPERIMENT Russian patent published in 2011 - IPC A61B10/00 A61K35/28 A61K38/20 A61K39/395 G01N33/53 G09B23/28 

FIELD: medicine.

SUBSTANCE: invention relates to biology and medicine, namely to experimental models of immunologic states, and can be used for estimation of anti-ergotypic response to vaccination with polyclonally activated cells. For this purpose vaccination with splenocytes, activated by means of anti-CD3 antibodies and interleukin-2, by subcutaneous injections 1 time per week during 4 weeks. After that response of delayed-type hypersensitivity (DTH) is induced by introduction of activated splenocytes under aponeurosis of animal paw pad. Anti-ergotypic response is estimated by degree of DTH development.

EFFECT: implementation of method provides possibility of experimental reliable estimation of anti-ergotypic response in vivo, induced by polyclonally activated cells, and estimate its connection with changes of autoimmune disease course, performing control over autoimmune responses.

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