METHOD OF PREPARING CULTURE MEDIUM FOR COUNTING SAPROPHYTIC HETEROTROPHIC BACTERIA IN SEA WATER Russian patent published in 2011 - IPC C12N1/20 C12Q1/04 C12R1/01 

FIELD: chemistry.

SUBSTANCE: culture base of a natural sea medium is mixed with microbiological agar and sterile sea water collected from a region where saprophytic heterotrophic bacteria are released. A culture base in form of sterile broth of Laminaria Japonica seaweed is prepared. 100 g of the Laminaria Japonica is crushed. 500 ml of boiling water is added and the mixture is boiled for 3-5 minutes. The broth is filtered and twice sterilised with flowing steam at 100°C for 30 minutes. In order to prepare 1 l of the culture medium, the starting components are taken with the following content of dry substances, g/l: Laminaria Japonica broth 0.31-1.24, microbioligical agar 10-15, sea water - up to 1 l.

EFFECT: high efficiency of the culture medium and simple method of preparing said culture medium.

1 tbl, 5 ex