FIELD: medicine.
SUBSTANCE: polypeptide contains extracellular and transmembrane domains of a human tissue factor. A DNA fragment coding this polypeptide, and a plasmid pET28a(+) are used to produce a genetic make-up enabling biosynthesis of the recombinant human tissue factor. Also, the E coli BL21 [DE3]/p6E-tTF strain that is a producer of the recombinant human tissue factor is offered.
EFFECT: high-yield recombinant protein and simplified recovery and purification procedures.
2 cl, 4 dwg, 3 ex
| Title |
Year |
Author |
Number |
| EXPRESSION PLASMID CONTAINING DNA FRAGMENT CODING MUTEIN [C245S] OF HUMAN TISSUE FACTOR WITH INSEPARABLE C-TERMINAL TAG, BACTERIUM E coli PRODUCING PRECURSOR PROTEIN OF MUTEIN [C245S] OF HUMAN TISSUE FACTOR WITH INSEPARABLE C-TERMINAL TAG, METHOD FOR PRODUCING RECOMBINANT MUTEIN [C245S] OF HUMAN TISSUE FACTOR WITH INSEPARABLE C-TERMINAL TAG, RECOMBINANT MUTEIN [C245S] OF HUMAN TISSUE FACTOR WITH INSEPARABLE C-TERMINAL TAG |
2012 |
- Vorob'Ev Ivan Ivanovich
- Orlova Nadezhda Aleksandrovna
- Usakin Lev Alekseevich
- Matskevich Viktor Aleksandrovich
|
RU2550027C2 |
| RECOMBINANT PLASMID PET21-TF7, PROVIDING SYNTHESIS OF MODIFIED TISSUE FACTOR, AND ESCHERICHIA COLI BL21 (DE3) PET21-TF7 STRAIN - PRODUCER OF RECOMBINANT HUMAN TISSUE FACTOR |
2020 |
- Shcherbakov Dmitrij Nikolaevich
- Elchaninov Vadim Valentinovich
- Shanshin Daniil Vasilevich
- Balabova Dina Vladimirovna
- Koval Anatolij Dmitrievich
- Erin Dmitrij Nikolaevich
|
RU2744592C1 |
| PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF INACTIVE PRECURSOR OF MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, PRECURSOR OF RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, METHOD OF PRODUCTION OF RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN, RECOMBINANT MUTEIN [C112S] OF HUMAN ENTEROKINASE LIGHT CHAIN |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
- Matskevich Viktor Aleksandrovich
- Zvjagin Ivan Vladimirovich
|
RU2495934C2 |
| PLASMID FOR EXPRESSION IN BACTERIA CELLS OF GENUS Escherichia OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TISSUE PLASMINOGEN ACTIVATOR (TPA) (RETEPLASE), BACTERIA BELONGING TO GENUS Escherichia, - PRODUCER OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) OR [-1] METHIONYL - FRAGMENT OF HUMAN TPA (RETEPLASE), PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE), METHOD OF PRODUCTION OF PRECURSOR OF RECOMBINANT FRAGMENT OF HUMAN TPA (RETEPLASE) |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
|
RU2495933C2 |
| HUMAN PLASMIDE pBSH2EGF DNA ENCODING SYNTHESIS OF HUMAN EPIDERMAL GROWTH FACTOR AND METHOD FOR PRODUCTION WHEREOF USING THE SAME |
2004 |
- Bykov Valerij Alekseevich
- Solov'Ev Andrej Ivanovich
- Nikolaenko Tat'Jana Vital'Evna
- Chumakov Artem Andreevich
- Lutsenko Elena Valer'Evna
|
RU2289623C2 |
| PLASMID FOR EXPRESSION IN CELLS OF BACTERIUM BELONGING TO Escherichia CLASS, NON-ACTIVE PREDECESSOR OF DNase I OF HUMAN OR ITS MUTEINS; BACTERIUM BELONGING TO Escherichia CLASS, - PRODUCER OF NON-ACTIVE PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING CONJUGATES OF POLYETHYLENE GLYCOL AND RECOMBINANT MUTEIN OF HUMAN DNase I, FERMENTATIVE ACTIVE CONJUGATE OF MUTEIN OF RECOMBINANT DNase I OF HUMAN |
2011 |
- Orlova Nadezhda Aleksandrovna
- Vorob'Ev Ivan Ivanovich
|
RU2502803C2 |
| RECOMBINANT PLASMID DNA PET3.54, ENCODING POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR |
2013 |
- Shingarova Ljudmila Nikolaevna
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2531547C1 |
| RECOMBINANT PLASMID DNA pET-32a, CODING GEN LIGNAD-BINDING DOMAIN OF RECEPTOR II TYPE OF HUMAN TRANSFORMING GROWTH FACTOR-β (TβRII), STRAIN OF BACTERIA Escherichia coli - PRODUCENT OF FUSION PROTEIN THIOREDOXIN/TβRII AND METHOD OF RENATURATION AND PURIFICATION OF TARGET PROTEIN TβRII |
2009 |
- Gasparjan Marine Ehduardovna
- Elistratov Pavel Alekseevich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2413769C1 |
| RECOMBINANT PLASMID DNA pFGM17 ENCODING POLYPEPTIDE OF HUMAN GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR AND STRAIN OF BACTERIUM Escherichia coli BL21(DE3)/pFGM17 AS PRODUCER OF POLYPEPTIDE OF HUMAN GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR |
2004 |
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Shingarova Ljudmila Nikolaevna
- Kirpichnikov Mikhail Petrovich
|
RU2271392C1 |
| FUSED PROTEIN OF THIOREDOXIN AND INFESTIN 4 DOMAIN, METHOD FOR PREPARING IT, EXPRESSION PLASMID DNA CODING FUSED PROTEIN, AND BACTERIUM Escherichia coli TRANSFORMED BY THIS PLASMID DNA |
2012 |
- Vorob'Ev Ivan Ivanovich
- Orlova Nadezhda Aleksandrovna
- Koljadko Vladimir Nikolaevich
|
RU2528251C2 |
