PLASMID FOR EXPRESSION IN CELLS OF BACTERIUM BELONGING TO Escherichia CLASS, NON-ACTIVE PREDECESSOR OF DNase I OF HUMAN OR ITS MUTEINS; BACTERIUM BELONGING TO Escherichia CLASS, - PRODUCER OF NON-ACTIVE PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; PREDECESSOR OF RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING RECOMBINANT DNase I OF HUMAN OR ITS MUTEIN; METHOD FOR OBTAINING CONJUGATES OF POLYETHYLENE GLYCOL AND RECOMBINANT MUTEIN OF HUMAN DNase I, FERMENTATIVE ACTIVE CONJUGATE OF MUTEIN OF RECOMBINANT DNase I OF HUMAN Russian patent published in 2013 - IPC C12N15/63 C12N15/12 C07K14/47 C12R1/185 

FIELD: biotechnologies.

SUBSTANCE: invention represents a method for obtaining recombinant DNAse I of a human or its mutein, as well as their conjugates with polyethylene glycol, using a bacterium belonging to Escherichia class, transformed with expression plasmid, containing a promoter functioning in a bacterial cell, DNA fragment coding a hexahistidine cluster, a fragment coding enterokinase recognition sequence amalgamated in frame with human DNAse I or its functionally active mutein containing replacements of asparagine with cysteine, transcription termination section, vector pET28a(+) fragment containing initiation section of replication of bacteriophage fl, sequence coding aminoglycoside-3'-phosphotransferase, area of beginning of plasmid pBR322 replication, gene RNA-organising protein Rop, sequence coding lactose operon repressor.

EFFECT: invention allows obtaining recombinant human DNAse I or its mutein with high yield.

18 cl, 7 dwg, 1 tbl, 12 ex