FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, and represents a method of producing a recombinant mutein [C112S] of human enterokinase light chain, using a bacterium belonging to the genus Escherichia, transformed with an expression plasmid containing a DNA fragment encoding a mutein [C112S] inactive precursor of human enterokinase light chain, comprising a sequence encoding a cleavable N-terminal peptide comprising a hexahistidine cluster and the enterokinase recognition sequence, and a sequence fused with it in frame encoding a precursor of mutein [C112S] of human enterokinase light chain with non-cleavable C-terminal hexahistidine cluster under control of a promoter operating in the bacterial cell.
EFFECT: invention enables to produce the recombinant mutein of human enterokinase light chain with a high yield.
11 cl, 5 dwg, 1 tbl, 6 ex
| Title |
Year |
Author |
Number |
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|
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2011 |
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|
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2011 |
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|
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2008 |
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RU2426780C2 |
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2009 |
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