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IPC

C12N15/00(2006-01-01)

C12N1/20(2006-01-01)

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Application

2013109369/10, 2013-03-04

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Start date

2013-03-04

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Actual filing date

2013-03-04

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Published

2014-10-20

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Inventor

Shingarova Ljudmila NikolaevnaPetrovskaja Lada Evgen'EvnaKrjukova Elena AleksandrovnaBoldyreva Elena FilippovnaDolgikh Dmitrij AleksandrovichKirpichnikov Mikhail Petrovich

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Nauki Institut Biorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Rossijskoj Akademii Nauk Ran)

RECOMBINANT PLASMID DNA PET3.54, ENCODING POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR Russian patent published in 2014 - IPC C12N15/00 C12N1/20

Abstract RU 2531547 C1

FIELD: biotechnology.

SUBSTANCE: invention is a recombinant plasmid DNA pET3.54, encoding the polypeptide FN3.54, interacting with human tumour necrosis factor. The present invention also discloses the recombinant strain of bacteria Escherichia coli BL21(DE3)/pET3.54 - producer of the polypeptide FN3.54, interacting with human tumour necrosis factor.

EFFECT: invention enables to obtain the desired protein with high yield, using a smaller amount of the inducer and the expression level of total cell protein.

2 cl, 4 dwg, 6 ex

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RU 2 531 547 C1

Authors

Shingarova Ljudmila Nikolaevna

Petrovskaja Lada Evgen'Evna

Krjukova Elena Aleksandrovna

Boldyreva Elena Filippovna

Dolgikh Dmitrij Aleksandrovich

Kirpichnikov Mikhail Petrovich

Dates

2014-10-20—Published

2013-03-04—Filed