METHOD OF PRODUCING STEROID DERIVATIVES BY OXASTEROID REDUCTION OR HYDROXYSTEROID OXIDATION WITH USING HYDROXYSTEROID DIHYDROGENASE Russian patent published in 2011 - IPC C12P33/02 C12N9/04 

FIELD: medicine.

SUBSTANCE: there is offered a method of enantio-selective enzymic reduction of oxasteroid compounds of general formula I and formula II presented in the invention description. The oxasteroid compound in a reaction mixture in the concentration ≥50 g/l is reduced by hydroxysteroid dihydrogenase in the presence of a NADH or NADPH cofactor. The oxidised NAD or NADP cofactor produced by hydroxysteroid dihydrogenase is continuously reduced through secondary alcohol oxidation or through C₄-C₆-cycloalkanol oxidation. For secondary alcohol or cycloalkanol oxidation, complementary oxydoreductase/alcohol dehydrogenase is used. Also, there is offered a method of enantio-selective enzymic oxidation of hydroxysteroid compounds of general formula I or hydroxysteroid compounds which are derivatives of bile acid. The hydroxysteroid compound in a reaction mixture in the concentration ≥50 g/l is oxydated by hydroxysteroid dihydrogenase in the presence of NADH or NADPH cofactor. The reduced NADH or NADPH cofactor produced by hydroxysteroid dihydrogenase is continuously regenerated through keto compound reduction or through C₄-C₆-cycloalkanol reduction. For keto compound or cycloalkanol reduction, complementary oxydoreductase/alcohol dehydrogenase is used. The inventions enable oxidation/reduction of initial hydroxysteroid/oxasteroid compounds of yield exceeding 90 % and higher TTN cofactors > 10³.

EFFECT: improved results.

18 cl, 6 ex