FIELD: chemistry.
SUBSTANCE: invention relates to a method for the enzyme regeneration of reductive-oxidative cofactors NAD+/NADH and/or, in particular, NADP+/NADPH in a joint reaction in which, result from at least two enzyme catalyzed reductive-oxidative reactions taking place in one reaction mass (product formation reactions), one of the two redox cofactors is accumulated in a reduced form and the other, respectively, in an oxidized form, a) in the regeneration reaction wherein the reduced cofactor is converted to the original oxidized form, oxygen or a compound of general formula is reduced, where R1 is a linear or branched (C1-C4)-alkyl group or (C1-C4)-carboxyalkyl group, and b) in the regeneration reaction, wherein the oxidized cofactor is converted to the original reduced form, (C4-C8)-cycloalkanol or a compound of general formula
is oxidized, where R2 and R3 are independently selected from the group consisting of H, (C1-C6) alkyl, wherein the alkyl is linear or branched, (C1-C6) alkenyl, where the alkenyl is linear or branched and contains from one to three double bonds, aryl, in particular C6-C12 aryl, carboxyl, or (C1-C4) carboxyalkyl, in particular, also cycloalkyl, for example C3-C8 cycloalkyl, where the oxidation reaction(s) and the reduction reaction(s) proceed on the same basic molecular chain.
EFFECT: method for redox cofactors regeneration was developed for cost-effective implementation of two or more enzyme catalyzed reductive-oxidative reactions in one reaction mass.
14 cl, 11 dwg, 13 ex
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Authors
Dates
2017-11-09—Published
2013-02-06—Filed