METHOD FOR ENZYME REGENERATION OF REDUCTIVE-OXIDATIVE COFACTORS Russian patent published in 2017 - IPC C12P19/02 C12P19/36 C12P33/00 C12P41/00 C13K11/00 

FIELD: chemistry.

SUBSTANCE: invention relates to a method for the enzyme regeneration of reductive-oxidative cofactors NAD⁺/NADH and/or, in particular, NADP⁺/NADPH in a joint reaction in which, result from at least two enzyme catalyzed reductive-oxidative reactions taking place in one reaction mass (product formation reactions), one of the two redox cofactors is accumulated in a reduced form and the other, respectively, in an oxidized form, a) in the regeneration reaction wherein the reduced cofactor is converted to the original oxidized form, oxygen or a compound of general formula is reduced, where R₁ is a linear or branched (C₁-C₄)-alkyl group or (C₁-C₄)-carboxyalkyl group, and b) in the regeneration reaction, wherein the oxidized cofactor is converted to the original reduced form, (C₄-C₈)-cycloalkanol or a compound of general formula is oxidized, where R₂ and R₃ are independently selected from the group consisting of H, (C₁-C₆) alkyl, wherein the alkyl is linear or branched, (C₁-C₆) alkenyl, where the alkenyl is linear or branched and contains from one to three double bonds, aryl, in particular C₆-C₁₂ aryl, carboxyl, or (C₁-C₄) carboxyalkyl, in particular, also cycloalkyl, for example C₃-C₈ cycloalkyl, where the oxidation reaction(s) and the reduction reaction(s) proceed on the same basic molecular chain.

EFFECT: method for redox cofactors regeneration was developed for cost-effective implementation of two or more enzyme catalyzed reductive-oxidative reactions in one reaction mass.

14 cl, 11 dwg, 13 ex