METHOD OF DIAGNOSING KIDNEY ALLOTRANSPLANT REJECTION Russian patent published in 2011 - IPC G01N33/49 

FIELD: medicine.

SUBSTANCE: method of diagnosing rejection of kidney allotransplant includes determination of phase height PH of peripheral blood live lymphocytes by method of phase-interference microscopy, determination of quantity of lymphocytes with phase height PH≤1.5 mcm, 1.5 mcm<PH≤2 mcm, 2 mcm<PH≤2.5 mcm, PH>2.5 mcm, selection of lymphocyte activity coefficients for each limit, said phase heights of lymphocytes equal k₃=3, k₂=2, k₁=1, k₀=0 respectively. Obtained data are used to determine functional activity of lymphocytes in sample by formula: FA=(k₃n₃+k₂n₂+k₁n₁+k₀n₀)/n, where n is number of lymphocytes in sample, n₃ is number of lymphocytes with PH≤1.5 mcm, n₂ is number of lymphocytes with 1.5 mcm<PH≤2 mcm, n₁ is number of lymphocytes with 2 mcm<PH≤2.5 mcm, n₀ is number of lymphocytes with PH>2.5 mcm, k₃, k₂, k₁, k₀ are coefficients of lymphocyte activity, and if value of lymphocyte functional activity is within FA=1.8-2.0, rejection of kidney transplant is diagnosed.

EFFECT: application of claimed method makes it possible to set diagnosis in due time, which considerably increases efficiency of anti-crisis therapy in post-transplantation period.

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