FIELD: medicine.
SUBSTANCE: method for producing erythrocytic membranes, consisting that haemolysis is enabled by erythrocyte mass preliminary incubated in an equal volume of Krebs-Henseleit buffer pH 7.5 containing 20 % of DMSO, then frozen and stored under certain conditions; before the examinations, the samples are defrozen that is followed by haemolysis of the erythrocyte mass to 0.4 M in a trisHCl solution with centrifugal sedimentation of the membranes.
EFFECT: erythrocytic membranes produced by the method described above preserve their morphofunctional status.
1 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR EVALUATING HEMOLYTIC ACTION OF POLYMERS, POLYMER MATERIALS AND ARTICLES MADE FROM THEM IN VITRO IN STATIC CONDITIONS | 2023 |
|
RU2818010C1 |
| METHOD FOR DETECTING VELOCITY IN DEVELOPMENT OF LIPID PEROXIDATION PRODUCTS IN ERYTHOCYTIC MEMBRANES IN FARM ANIMALS | 2002 |
|
RU2229133C1 |
| METHOD OF PRODUCTION OF HAEMOGLOBIN SOLUTIONS PURIFIED OF STROMAL COMPONENTS | 2006 |
|
RU2329826C1 |
| METHOD FOR DETERMINING ACID STABILITY OF ERYTHROCYTES COLD-BLOODED ANIMALS | 2014 |
|
RU2571081C1 |
| THE SUBSTANCE POSSESSING AN ANTICALCIUM ACTIVITY | 0 |
|
SU1836087A3 |
| METHOD FOR DETERMINING THE CONDITION OF A LIPID COMPONENT OF A CELL MEMBRANE | 2019 |
|
RU2717248C1 |
| METHOD OF DETERMINING OSMOTIC RESISTANCE OF ERYTHROCYTES | 2014 |
|
RU2580300C1 |
| METHOD FOR IDENTIFYING PROTECTIVE ACTION OF MICROORGANISMS ON A HEMOGLOBIN MOLECULE | 2017 |
|
RU2687061C2 |
| METHOD OF DETERMINATION THE SUITABILITY OF THE CANNED ERYTHROCYTIC SUSPENSION FOR TRANSFUSION | 2017 |
|
RU2647457C1 |
| METHOD FOR DETERMINING GLYCOSYLATED HEMOGLOBIN IN BLOOD | 1993 |
|
RU2038597C1 |
