FIELD: medicine.
SUBSTANCE: invention refers to medicine and can be used to determine the condition of the lipid component of cell membranes. That is ensured by blood sampling, centrifugation and washing of erythrocyte cells with physiologic saline. Erythrocytes are mixed with acetonitrile at ratio of 1:1. Mixture is centrifuged at 3,000 rpm for 30 minutes. Supernatant is then taken into a tray and distilled water is added at ratio of 1:9. Spectrophotometry is then performed at 215 nm and 235 nm. Values of lipid oxidation (Vlo) are determined. With a Vlo value equal to or less than 0.43±0.04, normal state of the lipid component of the cell membrane is determined. At value of Vlo more than 0.43±0.04 make conclusion on lipid component disorder of cell membrane.
EFFECT: invention enables assessing the condition of the lipid component of the cell membrane and detecting its disorganization in developing pathological processes in the patients.
1 cl, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR INVESTIGATING METABOLISM PROCESS ACTIVITY IN CELLS | 1992 |
|
RU2050001C1 |
| METHOD FOR ASSAY OF STABILITY OF ERYTHROCYTE CELLULAR MEMBRANE | 2002 |
|
RU2234088C2 |
| METHOD FOR ASSESSING ERYTHROCYTE MEMBRANE MICROVISCOSITY BY CALCULATING PYRENE EXIMERISATION COEFFICIENT C IN PREGNANT WOMEN SUFFERED AGGRAVATED HERPES VIRAL INFECTION IN THIRD TRIMESTER OF PREGNANCY WITH REGARD TO EVALUATION OF PERCENTAGE OF OLEIC ACID IN ERYTHROCYTE MEMBRANES | 2010 |
|
RU2467334C2 |
| METHOD FOR ASSESSING DISTURBED HAEMOGLOBIN OXYGENATION WITH REDUCED MICROVISCOSITY OF PERIPHERAL ERYTHROCYTE MEMBRANE OF PREGNANT WOMEN IN THIRD TRIMESTER OF GESTATION AND AGGRAVATED CYTOMEGALOVIRUS INFECTION | 2013 |
|
RU2540425C1 |
| METHOD FOR DETERMINATING ACTION SIDE IN REFLEXOTHERAPY OF PREGNANCY UNBEARING | 0 |
|
SU1639652A1 |
| METHOD FOR DETERMINATION OF ERYTHROCYTE MEMBRANE STATE | 2005 |
|
RU2296992C1 |
| METHOD OF DETERMINING STRUCTURAL STATE OF ERYTHROCYTE MEMBRANE | 2013 |
|
RU2528909C1 |
| METHOD FOR ASSESSING DAMAGING EFFECT OF CYTOMEGALOVIRAL INFECTION ON MYRISTIC ACID CONTENT IN UMBILICAL CORD BLOOD OF NEWBORNS FROM MOTHERS SUFFERED CYTOMEGALOVIRUS INFECTION REACTIVATION IN THIRD TRIMESTER | 2017 |
|
RU2687971C1 |
| METHOD OF DETERMINING INTRACELLULAR ACIDITY | 2009 |
|
RU2412440C1 |
| METHOD FOR SELECTING THERAPY FOR CORRECTING LIPID METABOLISM DISORDERS IN CHRONIC PYELONEPHRITIS PATIENTS | 2005 |
|
RU2310864C2 |
