FIELD: medicine.
SUBSTANCE: method for producing an Escherichia coli cell fraction showing protease inhibiting activity provides bacteria cells preservation in the presence of buffered 80-90% glycerine, processing by 3% triton X-100 for removing a cell membrane. Produced cytoplasm proteins are extracted by the increasing salt concentration, namely 0.14 M, 0.35 M; 2 M NaCl, 6 M guanidine hydrochloride with 0.1% β-mercaptoethanole. It is followed by affine sepharose 4B chromatography with immobilised trypsin and estimation of protease inhibiting activity in eluates.
EFFECT: invention can be used in analysis of molecular-genetic mechanisms of procaryote cell structure formation and roles of protein components in their organisation, and also genome remodelling nature that is necessary for disclosing of regulation pathways of mechanisms of macro-and microorganism action, and also search of new drug targets and development of ecologically safe new drugs.
3 tbl, 4 dwg
