FIELD: biotechnologies.
SUBSTANCE: method includes the following stages: conservation of cells in presence of buffered 80-90% glycerine, collapse of cell shells by 3% triton X-100, extraction by increasing concentrations of salts: 0.14 M, 0.35 M; 2 M NaCl, 6 M guanidine hydrochloride with 0.1% β-mercaptoethanol, extraction of positively charged proteins from above fractions with the help of ion-exchange chromatography with amberlite IRC-50 in an interrupted gradient of guanidine hydrochloride: 6%, 8.9%, 10.6%, 13% on 0.1 M potassium-phosphate buffer pH 6.8 and detection of sites of sensitivity in them to Arg-X proteolysis.
EFFECT: invention may be used in analysis of molecular-genetic mechanisms of procaryote cell structure formation and role of protein components in their organisation, and also when studying features of genome remodelling, which is necessary for opening of paths of regulation mechanisms of macro- and microorganisms.
9 dwg, 1 ex
