STRAIN OF HYBRID CULTIVATED CELLS OF ANIMAL Mus musculur 1E6-PRODUCER OF MONOCLONAL ANTIBODIES, SPECIFIC TO BACILLUS Anthracis spores Russian patent published in 2012 - IPC C12N5/18 C12P21/08 C07K16/12 

FIELD: medicine.

SUBSTANCE: strain of hybridioma is obtained by immunization of mice of line BALB/c. Mice are immunised by conventional method by double with thirty day exposition subcutaneous introduction of inactivated spores of B. Anthracis strain "СТИ-1". On the third day after last buster-injection carried out is hybridisation of splenocytes of immune mice (1×10⁸ cells) with cells of mouse myeloma P3-X63 Ag/8-653 (1×10⁷ cells), using for fusion polyethylene glycol (Sigma, USA). After hybridisation carried out are selection, screening, cloning and cryopreservation of hybridoma. Obtained hybridoma is deposited in collection of microorganisms of FSSE SRC AMB under number H10. Invention relates to biotechnology and can be used for obtaining monoclonal antibodies (MCA) to spores Bacillus anthracis. Strain productivity is 20-50 mcg/ml of monoclonal antibodies (MCAla in ascitic liquid- 5-10 mg/ml. Hybridoma produces MCA, related to IgGl subclass of mouse immunoglobulins and spores specific to Bac.anthracis.

EFFECT: increase of strain productivity.

7 dwg, 2 tbl, 9 ex