FIELD: biotechnology.
SUBSTANCE: disclosed is a strain of hybrid cultured H.sapiens / Mus musculus 8D4E9-Ba-LF cells, which produces human monoclonal antibodies against lethal factor of anthrax. Strain is deposited in "GKPM-Obolensk", collection number N-79. Produced antibodies are capable of neutralizing the lethal toxin Bacillus anthracis on a cell model in vitro and in vivo when introducing into laboratory mice a lethal toxin Bacillus anthracis composed of recombinant proteins of a protective antigen and a lethal factor with previous passive immunization of the animals with the declared antibodies. Administration of antibodies increases survival rate of animals in passive immunization.
EFFECT: use of the invention enables producing monoclonal antibodies capable of neutralizing a lethal toxin of an anthrax agent which can be used further as passive protection of a human in case of infection or potential infection with anthrax.
1 cl, 6 dwg, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| HOMO SAPIENS/MUS MUSCULUS 1B9C7 HYBRID CULTURED CELL STRAIN: PRODUCER OF HUMAN MONOCLONAL ANTIBODIES SPECIFIC TO THE PROTEOLYTIC DOMAIN OF BOTULINUM TYPE A TOXIN | 2021 |
|
RU2783897C1 |
| THE STRAIN OF HYBRID CULTURED HOMO SAPIENS/Mus MUSCULUS CELLS Hu-C6D7-RBD IS A PRODUCER OF HUMAN MONOCLONAL ANTIBODIES SPECIFIC TO THE RBD DOMAIN S OF THE SARS-CoV-2 VIRUS PROTEIN | 2022 |
|
RU2788359C1 |
| METHOD OF DETECTING PRESENCE OF ANTHRAX INFECTION IN BIOLOGICAL FLUIDS AND ENVIRONMENT | 2009 |
|
RU2418860C1 |
| 1E10 MONOCLONAL MOUSE ANTIBODY 1E10 AND RECOMBINANT CHIMERIC (MOUSE-HUMAN) ANTIBODY XI1E10, NEUTRALIZING BACILLUS ANTHRACIS LETHAL TOXIN, AND A STRAIN OF HYBRID CULTURED ABIMAL CELLS -MUS MUSCULUS 1E10 | 2020 |
|
RU2745116C1 |
| METHOD FOR IMMUNOFLUORESCENT DETECTION OF PROTECTIVE ANTIGEN OF ANTHRAX AGENT | 2011 |
|
RU2478970C1 |
| STRAIN OF HYBRID CULTIVATED CELLS OF ANIMAL Mus musculur 1E6-PRODUCER OF MONOCLONAL ANTIBODIES, SPECIFIC TO BACILLUS Anthracis spores | 2010 |
|
RU2439148C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus 2B8 - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO Yersinia pestis V ANTIGEN | 2011 |
|
RU2478704C1 |
| METHOD FOR DETERMINING ANTHRAX LETHAL FACTOR PROCEEDING FROM IMMUNODETECTION ASSOCIATED WITH POLYMERASE CHAIN REACTION | 2011 |
|
RU2486524C1 |
| STRAIN OF HYBRID ANIMAL CELLS Mus musculus 5G6 - PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO Yersinia pestis V ANTIGEN | 2011 |
|
RU2478703C1 |
| ANIMAL MUS MUSCULUS HYBRID CELL F3H10 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES | 2009 |
|
RU2407795C1 |
