METHOD OF OBTAINING MONOCLONAL ANTIBODIES TO ABLUMINAL MEMBRANE ANTIGEN OF CEREBRAL ENDOTHELIOCYTES Russian patent published in 2012 - IPC C12P21/08 C07K16/18 

FIELD: medicine.

SUBSTANCE: mouse of line Balb/C is immunised with preparation of membrane proteins of cerebral endotheliocytes. B-lymphocytes of said mouse spleen are isolated and fused with cells of myeloma of line Sp2/0-Ag14 mouse, hybridomas are obtained, supernatants of obtained hybridomas are tested in immune-chemical way and hybridomas, producing monoclonal antibodies to abluminal membrane antigen of cerebral endotheliocytes, are separated. From the selected is hybridoma, characterised by the largest number of antibody-producing hybrid cells, synthesising antibodies belonging to class of G immunoglobulins preserving biological activity in blood serum for not less than 24 hours, monoclonal antibodies are separated from supernatant of selected hybridoma, in particular, by method of affine chromatography on protein-G-agarose.

EFFECT: method by invention ensures obtaining monoclonal antibodies to antigen of cerebral endotheliocytes with high immune-chemical activity, making it possible to visualise cerebral endotheliocytes both ex vivo, and in conditions in vitro and in vivo for characteristics of vascular system of gliomas.

2 cl, 9 dwg, 1 ex