VITRO DIAGNOSTIC KIT FOR IDENTIFICATION OF HUMAN PAPILLOMAVIRUS IN CLINICAL SAMPLES Russian patent published in 2012 - IPC C12Q1/68 C12P19/34 C12N15/11 

Abstract RU 2441918 C2

FIELD: medical technology.
SUBSTANCE: invention relates to the area of biotechnology, namely to the method for detection and typing of human papillomavirus (HPV) in a sample, as is a reaction vessel. The invention also relates to apparatus for use in the kit and the HPV detection and typing method. The assay comprises: performing a nucleic acid amplification reaction on a sample, the amplification reaction being intended to amplify an HPV target sequence in a non-type specific manner; obtaining single stranded oligonucleotides from any amplification products; allowing single stranded oligonucleotides to hybridise where possible with the HPV type-specific probes chosen from the group containing SEQ ID NO:1 - SEQ ID NO:133 and chosen according to their type, as given in table 1, provided on a solid support, the support being located within a reaction vessel suitable for containing the sample; and detecting hybridised oligonucleotides if HPV is present in the sample.

EFFECT: specific identification of the HPV types if HPV is present in a sample.

63 cl, 7 dwg, 2 tbl, 3 ex

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RU 2 441 918 C2

Authors

Gaskon Ehskobar Irene

Vill'Jaehrmosa Khaehn Marija Luisa

Dates

2012-02-10Published

2006-08-04Filed