FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology and virology. The hMPV amplification according to the present invention is compatible with detecting the other viruses causing respiratory infections, as well as with the hMPV genetic typing as found in a sample. After the test sample are amplified with primers specific for each virus, the amplification products are used to produce one-chain DNAs and hybridised with the probes specific to the relevant viruses.
EFFECT: there are described the method and kit for detecting and identifying human metapneumovirus (hMPV) in the test samples, as well as s number of sequences and their application as the hMPV amplification primers.
19 cl, 5 dwg, 2 tbl, 2 ex
Title | Year | Author | Number |
---|---|---|---|
SETS OF OLIGONUCLEOTIDE PRIMERS AND PROBES, BIOLOGICAL MICROCHIP AND TEST SYSTEM FOR IDENTIFYING AND TYPING INFLUENZA A AND B VIRUS WITH USING THEM | 2013 |
|
RU2538168C2 |
COMPOSITIONS AND METHODS FOR DETECTION AND IDENTIFICATION OF NUCLEIC ACID SEQUENCES IN BIOLOGICAL SAMPLES | 2012 |
|
RU2595421C2 |
ANALYSIS OF RESPIRATORY INFECTION | 2012 |
|
RU2642304C2 |
INCREASING VARIABLE-FREE PARTICLES OUTPUT IN PLANTS | 2012 |
|
RU2682752C2 |
NEISSERIA MENINGITIDIS POLYPEPTIDES | 2007 |
|
RU2450019C2 |
SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
|
RU2818960C1 |
OBTAINING VIRUS-LIKE PARTICLES OF INFLUENZA VIRUS IN PLANTS | 2014 |
|
RU2742607C1 |
OBTAINING VIRUS-LIKE PARTICLES OF INFLUENZA VIRUS IN PLANTS | 2014 |
|
RU2705555C2 |
METHOD FOR DETECTION OF HERPES VIRUS IN TESTED SAMPLE | 2009 |
|
RU2470999C2 |
SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND A FLUORESCENCE-LABELED PROBE FOR IDENTIFICATION OF HUMAN CORONAVIRUS 2019-nCoV RNA BY PCR WITH HYBRIDIZATION-FLUORESCENCE DETECTION IN REAL TIME | 2020 |
|
RU2734300C1 |
Authors
Dates
2013-04-10—Published
2009-05-27—Filed