FIELD: medicine.
SUBSTANCE: what is described is fatty acid synthetase of a sequence presented in the description. What is specified is a polynucleotide coding such synthetase. What is presented is a vector containing such polynucleotide. Besides, what is also described is a transformed microbial organism containing such vector or polynucleotide. The transformed microbial organism is used to produce fatty acids. What is offered is a method for producing a lipid or a fatty acid that involves transformant cell culture followed by recovering the lipid or the fatty acid from such cells. What is described is a method for assessment of ability to form fatty acids based on the measurement of an expression level of fatty acid synthetase having a nucleotide sequence of specified polynucleotide. Also, there is presented a method for selection of microbial lipid-producing organisms that is based on the comparison of the expression levels of specified gene in a reference and tested microbial organism.
EFFECT: invention provides producing high-yield fatty acids.
17 cl, 1 dwg, 2 tbl, 1 ex
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